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#27901 Human secreted α-Klotho Assay Kit - IBL

  • #27901 Human secreted α-Klotho ELISA Kit
  • #27901 Human secreted α-Klotho ELISA Kit
  • #27901 Human secreted α-Klotho ELISA Kit
  • #27901 Human secreted α-Klotho ELISA Kit
  • #27901 Human secreted α-Klotho ELISA Kit
Intended Use：
Research reagents
Measuring Method：
ELISA
Sample Types：
Human
Measuring Samples：
Serum, EDTA Plasma, Cerebrospinal fluids
Measurement Range：
70.31 - 4,500 pg/mL
Package Size1：
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
　 for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
　 enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 27901
Product Name Human secreted α-Klotho Assay Kit - IBL
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human
Measuring Samples Serum, EDTA Plasma, Cerebrospinal fluids
Measurement Range 70.31 - 4,500 pg/mL
Primary Reaction Overnight at 2 - 8 ℃
Secondary Reaction 60 minutes at 2 - 8 ℃
Sensitivity 9.87 pg/mL
Specificity Substance Cross reactivity (%)
Human secreted α-Klotho 100%
Human β-Klotho ≦0.1%
Human transmembrane α-Klotho Isoform ≦0.1%
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Available
Package Size 1 96 Well

Product Description

Product Description

α-Klotho (Alpha Klotho) was identified as an extremely down-regulated gene in the genetically-modified mouse of which phenotype is very similar to various symptoms of human aging. The sequences of Alpha Klotho genes have been identified in various species including humans based on the one of mouse. Alpha Klotho protein is a 130 kDa, one-transmembrane protein and its expression is confirmed in the kidneys and the parathyroid glands. In recent years, it has become clear that Alpha Klotho is an important molecule within a living organism regulating the metabolism of mineral such as calcium and phosphorus. It is considered that in Alpha Klotho mouse, early aging-like symptoms are induced by impaired mineral homeostasis caused by decreased expression of Alpha Klotho. Meanwhile, it is reported that the long N-terminal extracellular domain which comprises the major portion of sequence of Alpha Klotho protein is released free into blood by shedding.
There is also a secreted Alpha-Klotho that has only first repeat due to gene splicing.
However, there are many unclear points regarding the function of secreted Alpha-Klotho protein and its concentration fluctuation, so it was required to develop the measurement system of Alpha Klotho. This kit specifically measures human secreted isoforms (secreted Alpha-Klotho) and does not react with soluble Alpha Klotho proteins derived from membrane Alpha Klotho.



Keywords:
α-Klotho / Alpha Klotho / FGF23 / Kidney disease / Chronic Kidoeny disease (CKD) / Mineral Bone Disease (MBD) / Osteoporosis / Aging / Alzheimer's Disease　

FAQ

FAQ

  • Question
    Q.Can mouse secreted α-Klotho be measured by this ELISA kit?
  • Answer
    A.No, it cannot.
  • Question
    Q.Can secreted α-Klotho in supernatant can be detected by this kit?
  • Answer
    A.Yes, it can be detected.
  • Question
    Q.What is the difference between soluble and secreted α-Klotho kits?
  • Answer
    A.The α-Klotho protein has been reported to shedding its long N-terminal extracellular domain, which occupies most of the sequence, and to be released in the blood (soluble). At the same time, gene splicing has been reported to produce a secreted isoform with only the first repeat (secreted).
    #27998 Human soluble α-Klotho Assay Kit - IBL specifically detects all soluble α-Klotho including the KL1 domain.
    #27901 Human secreted α-Klotho Assay Kit - IBL specifically detects secreted α-Klotho in the KL1 domain, including the sequence QVSQLTKPISSLTKPYH.
  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the Test sample value is influenced by lack of washing process or other operations. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
  • Question
    Q.What is the definition of Over Night (O/N) reaction?
    ELISA common FAQ
  • Answer
    A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.
  • Question
    Q.What is the specification of quality control for ELISA product release?
    ELISA common FAQ
  • Answer
    A.The information of specification is available on individual lot specific CoA. Please contact us with your reference lot number for obtaining of specific CoA.
  • Question
    Q.What is the number (e.g. 432143214321) at the edge of strips of the plate?
    ELISA common FAQ
  • Answer
    A.According to the plate maker (ThermoFisher), it does not have any specific meaning as it is just the number of molds.
  • Question
    Q.How to wash an ELISA plate?
    ELISA common FAQ
  • Answer
    A.Washing it by an auto-washer is highly recommended.
    If it is not available, please refer to the demo video (only 2 mins) using a washing bottle.
  • Question
    Q.The wells turned black during the test with the kit.
    ELISA common FAQ
  • Answer
    A.It is possible that the wells were not washed sufficiently during the washing process after the HRP-labeled antibody reaction.
    Be sure to wash the wells enough times as described in the data sheet with washing buffer of more than 350 µL.
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