#27268 Human Lipoprotein Lipase (LPL) Assay Kit - IBL

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Intended Use：: Research reagents

Measuring Method：: ELISA

Sample Types：: Human

Measuring Samples：: Serum, EDTA plasma, Heparin plasma, Post heparin EDTA plasma

Measurement Range：: 0.02 - 1.5 ng/mL

Package Size1：: 96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
　 for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
　 enclosed in the product purchased before use.

Product Overview

Product Code	27268
Product Name	Human Lipoprotein Lipase (LPL) Assay Kit - IBL
Maker Name	Immuno-Biological Laboratories Co., Ltd.
Intended Use	Research reagents
Measuring Method	ELISA
Conjugate	HRP
Species	Human
Measuring Samples	Serum, EDTA plasma, Heparin plasma, Post heparin EDTA plasma
Measurement Range	0.02 - 1.5 ng/mL
Primary Reaction	60 minutes at 37 ℃
Secondary Reaction	30 minutes at 2-8 ℃
Sensitivity	0.004 ng/mL
Specificity	Substance Cross reactivity (%) Human Lipoprotein Lipase（LPL） 100 Human Endothelial Lipase（EL） ≦0.1 Human Hepatic Triacylglycerol Lipase(HTGL) ≦0.1
Storage Condition	2 - 8 ℃
Poisonous and Deleterious Substances	Not Applicable
Cartagena	Not Applicable
Measuring Service	Not Available
Package Size 1	96 Well
Remarks1	This kit (27268) is an improved version of the older kit 27184, but there are no substantial differences between them in performance of the kit.

Product Description

LPL is an enzyme which catabolizes Triglyceride (TG) in triglyceride-rich lipoproteins such as dietary-derived Chylomicron and VLDL synthesized in the liver and it has an important role for TG metabolism with as the essential factor ApoC-II.

LPL is bound by GPIHBP1 or heparan sulfate proteoglycan on the surface of microvascular endothelium of adipose tissue rather than the liver or muscles.

Deficient of LPL and declining of its function are found in type I, IV and V hyperlipemia and it has been considered that they are one of the cause of high TG.

This kit can quantitatively measure LPL in human serum, EDTA plasma, heparin plasma and post heparin EDTA plasma.

References

Note: Retrieve by PMID number in displayed by abstract: http://www.ncbi.nlm.nih.gov

FAQ

Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
ELISA common FAQ
A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
Q.What is the composition of concentrated wash buffer?
ELISA common FAQ
A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
Q.What is the feature of the plate?
ELISA common FAQ
A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
Q.Can I re-use standard after reconstitution?
ELISA common FAQ
A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
Please check the details on each product datasheet.
Q.What is different between reagent blank and test sample blank?
ELISA common FAQ
A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the Test sample value is influenced by lack of washing process or other operations. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
Q.How many samples can be measured by this kit?
ELISA common FAQ
A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
Q.What is LOD (Limit of Detection)?
ELISA common FAQ
A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
Q.What is LOQ (Limit of Quantification)?
ELISA common FAQ
A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
Q.What is the definition of Over Night (O/N) reaction?
ELISA common FAQ
A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.
Q.What is the specification of quality control for ELISA product release?
ELISA common FAQ
A.The information of specification is available on individual lot specific CoA. Please contact us with your reference lot number for obtaining of specific CoA.
Q.What is the number (e.g. 432143214321) at the edge of strips of the plate?
ELISA common FAQ
A.According to the plate maker (ThermoFisher), it does not have any specific meaning as it is just the number of molds.
Q.How to wash an ELISA plate?
ELISA common FAQ
A.Washing it by an auto-washer is highly recommended.
If it is not available, please refer to the demo video (only 2 mins) using a washing bottle.
Q.The wells turned black during the test with the kit.
ELISA common FAQ
A.It is possible that the wells were not washed sufficiently during the washing process after the HRP-labeled antibody reaction.
Be sure to wash the wells enough times as described in the data sheet with washing buffer of more than 350 µL.