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#27267 Human GPIHBP1 Autoantibody Assay Kit - IBL

  • #27267 Human GPIHBP1 Autoantibody ELISA Kit
  • #27267 Human GPIHBP1 Autoantibody ELISA Kit
  • #27267 Human GPIHBP1 Autoantibody ELISA Kit
Intended Use：
Research reagents
Measuring Method：
ELISA
Sample Types：
Human
Measuring Samples：
Serum, EDTA-plasma, Heparin plasma, Post-heparin EDTA-plasma
Measurement Range：
0.008 - 0.500 U/mL
Package Size1：
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
　 for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
　 enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 27267
Product Name Human GPIHBP1 Autoantibody Assay Kit - IBL
Maker Name Immuno-Biological Laboratories Co., Ltd.
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human
Measuring Samples Serum, EDTA-plasma, Heparin plasma, Post-heparin EDTA-plasma
Measurement Range 0.008 - 0.500 U/mL
Primary Reaction 60 minutes at 25 ℃
Secondary Reaction 30 minutes at 25 ℃
Sensitivity 0.001 U/mL
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Not Available
Package Size 1 96 Well

Product Description

Product Description

GPIHBP1 (Glycosylphosphatidylinositol anchored high density lipoprotein binding protein 1) is an anchor protein that is modified by glycolipid and it has been known that it exists on a capillary endothelial cell membrane and involves with metabolism of triglyceride rich (TG-rich) lipoprotein (triglyceride). 
 

GPIHBP1 transports lipoprotein lipase (LPL) synthesized and secreted in adipocyte or skeletal muscle cells into capillary lumen by transcytosis and it binds LPL on the surface of endothelial cells so that GPIHBP1 has an critical role for metabolizing TG-rich lipoprotein.
 

It has been already know that type I hyperlipemia (hyperchylomicronemia) can be caused by GPIHBP1 gene mutation, however, the new insight regarding existence of GPIHBPI autoantibody as a novel disease entity has been reported on the following paper published by New England Journal of Medicine (NEJM).


Beigneux AP, Miyashita K, Ploug M, et al. Autoantibodies against GPIHBP1 as a Cause of Hypertriglyceridemia. N Engl J Med. 2017;376(17):1647-1658. doi:10.1056/NEJMoa1611930


According to the new insight, GPIHBP1 antibody against to GPIHBP1 protein interferes the binding between GPIHBP1 and LPL, then it blocks the transport of LPL by GPIHBP1. At the end, severe hyperchylomicronemia is caused by the phenomenon as same as gene mutation.


This kit can quantitatively measure GPIHBP1 autoantibody in human serum and EDTA-plasma samples.

References (Not listed at PubMed)
A Woman With HypertriglyceridemiaWho Acquired AntibodyAgainst GPIHBP1 Hirano et al. JACC:  CASE  REPORTS

References

References

Note: Retrieve by PMID number in displayed by abstract: http://www.ncbi.nlm.nih.gov

FAQ

FAQ

  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the Test sample value is influenced by lack of washing process or other operations. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
  • Question
    Q.What is the definition of Over Night (O/N) reaction?
    ELISA common FAQ
  • Answer
    A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.
  • Question
    Q.What is the specification of quality control for ELISA product release?
    ELISA common FAQ
  • Answer
    A.The information of specification is available on individual lot specific CoA. Please contact us with your reference lot number for obtaining of specific CoA.
  • Question
    Q.What is the number (e.g. 432143214321) at the edge of strips of the plate?
    ELISA common FAQ
  • Answer
    A.According to the plate maker (ThermoFisher), it does not have any specific meaning as it is just the number of molds.
  • Question
    Q.How to wash an ELISA plate?
    ELISA common FAQ
  • Answer
    A.Washing it by an auto-washer is highly recommended.
    If it is not available, please refer to the demo video (only 2 mins) using a washing bottle.
  • Question
    Q.The wells turned black during the test with the kit.
    ELISA common FAQ
  • Answer
    A.It is possible that the wells were not washed sufficiently during the washing process after the HRP-labeled antibody reaction.
    Be sure to wash the wells enough times as described in the data sheet with washing buffer of more than 350 µL.
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