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#27263 Human EL C-Terminal Assay Kit - IBL

  • #27263 Human EL C-Terminal ELISA Kit
  • #27263 Human EL C-Terminal ELISA Kit
  • #27263 Human EL C-Terminal ELISA Kit
  • #27263 Human EL C-Terminal ELISA Kit
  • #27263 Human EL C-Terminal ELISA Kit
Intended Use：
Research reagents
Measuring Method：
ELISA
Sample Types：
Human
Measuring Samples：
EDTA-plasma, Serum
Measurement Range：
0.13 - 8 ng/mL
Package Size1：
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
　 for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
　 enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 27263
Product Name Human EL C-Terminal Assay Kit - IBL
Maker Name Immuno-Biological Laboratories Co., Ltd.
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human
Measuring Samples EDTA-plasma, Serum
Measurement Range 0.13 - 8 ng/mL
Primary Reaction Overnight at 2 - 8 ℃
Secondary Reaction 30 minutes at 2 - 8 ℃
Sensitivity 0.01 ng/mL
Specificity Substance Cross Reactivity
Human EL C-Terminal 100%
Human EL Full-Length 100%
Human LPL ≦ 0.1%
Human HTGL ≦ 0.1%
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Available
Package Size 1 96 Well

Product Description

Product Description

Endothelial Lipase (EL) is a metabolic enzyme of HDL. It is phospholipase A1 molecule that is high substrate specificity against to phospholipid that exists in high density lipoprotein, HDL molecule and it promotes HDL metabolism through metabolizing HDL phospholipid.  It has been considered that EL involves with lipid metabolism and elevating risk of arteriosclerosis as strong expression of EL on newly formed vessel, macrophage in arteriosclerotic lesions is observed while EL expresses in vascular endothelial cells and vascular smooth myocytes of normal blood vessels.  It has been reported that both full length EL (active form) and cleaved EL (inactive forms, 40K fragment cleaved at N terminal and 28K fragment cleaved at C terminal cleaved by proprotein convertase) exist in blood. This ELISA kit is designed using two antibodies that detects C terminal fragments that can detect both full length EL and cleaved form EL that is cleaved at C-terminal in serum and EDTA-plasma regardless with or without administration of heparin injection that is conventionally required for the measurement.

The control set is available for sale on request. Learn More

Keywords:
Lipase / Lipid Metabolism / Dyslipidemia
 

FAQ

FAQ

  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the Test sample value is influenced by lack of washing process or other operations. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
  • Question
    Q.What is the definition of Over Night (O/N) reaction?
    ELISA common FAQ
  • Answer
    A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.
  • Question
    Q.What is the specification of quality control for ELISA product release?
    ELISA common FAQ
  • Answer
    A.The information of specification is available on individual lot specific CoA. Please contact us with your reference lot number for obtaining of specific CoA.
  • Question
    Q.What is the number (e.g. 432143214321) at the edge of strips of the plate?
    ELISA common FAQ
  • Answer
    A.According to the plate maker (ThermoFisher), it does not have any specific meaning as it is just the number of molds.
  • Question
    Q.How to wash an ELISA plate?
    ELISA common FAQ
  • Answer
    A.Washing it by an auto-washer is highly recommended.
    If it is not available, please refer to the demo video (only 2 mins) using a washing bottle.
  • Question
    Q.The wells turned black during the test with the kit.
    ELISA common FAQ
  • Answer
    A.It is possible that the wells were not washed sufficiently during the washing process after the HRP-labeled antibody reaction.
    Be sure to wash the wells enough times as described in the data sheet with washing buffer of more than 350 µL.
  • Question
    Q.How do you define the acceptance range of quality control of control sets?
    Control Set Common FAQ
  • Answer
    A.In principal, it is defined as ±15%-20% based the standard value described on each datasheet.
  • Question
    Q.What is the shelf life of control set?
    Control Set Common FAQ
  • Answer
    A.The shelf life is 12 months upon the goods is dispatched from our lab if the goods is unopned.
  • Question
    Q.What is the storage condition of control sets?
    Control Set Common FAQ
  • Answer
    A.It should be stored under chilled condition (2～10℃).
  • Question
    Q.Can the control set be used after reconstitution?
    Control Set Common FAQ
  • Answer
    A.No it cannot be re-used after the reconstitution.
  • Question
    Q.What is the package form of control sets?
    Control Set Common FAQ
  • Answer
    A.It is lyophilized form.
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