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#27709 Human Amyloidβ Toxic Oligomer Assay Kit - IBL
- Intended Use:
- Research reagents
- Measuring Method:
- ELISA
- Sample Types:
- Human
- Measuring Samples:
- CSF
- Measurement Range:
- 3.13 ~ 200 pg/mL
- Package Size1:
- 96 Well
※ The product indicated as "Research reagents" in the column Intended Use cannot be used
for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
enclosed in the product purchased before use.
Product Overview
Product Overview
Product Code | 27709 |
---|---|
Product Name | Human Amyloidβ Toxic Oligomer Assay Kit - IBL |
Maker Name | Immuno-Biological Laboratories Co., Ltd. |
Intended Use | Research reagents |
Measuring Method | ELISA |
Conjugate | HRP |
Species | Human |
Measuring Samples | CSF |
Measurement Range | 3.13 ~ 200 pg/mL |
Primary Reaction | Overnight at 2 - 8 ℃ |
Secondary Reaction | 60 minutes at 2 - 8 ℃ |
Sensitivity | 0.27 pg/mL |
Specificity | Compound Cross Reactivity Human Aβ (1-40) ≦0.1% Human Aβ (1-42) ≦0.1% |
Storage Condition | 2 - 8 ℃ |
Poisonous and Deleterious Substances | Not Applicable |
Cartagena | Not Applicable |
Measuring Service | Available |
Package Size 1 | 96 Well |
Product Description
Product Description
Oligomerization of Amyloidβ (Amyloid Beta) 42 is considered as an early event in Alzheimer ’s disease (AD). Irie et al. have proposed their hypothesis from the study of systematic proline scanning and solid-state NMR that Amyloid Beta conformer with a turn structure at positions 22 and 23 in aggregation of Amyloid Beta 42 is much high toxicity and it is also easily formed to become an oligomer.1) It has been considered that the toxic Amyloid Beta conformer is stable and easily formed to become a neurotoxic oligomer. The antibody (clone: 24B3) specifically detects a toxic Amyloid Beta conformer was established for development of the ELISA assay kit.2) This kit can measure selectively putative Amyloid Beta Oligomer in CSF.
The control set is available for sale on request. Learn More
Trend of Toxic Amyloidβ Oligomer Hypothesis
Related IBL News
Relationship between mild cognitive impairment (MCI) which suggests early stage of Alzheimer's disease and Amyloid β toxic oligomer –new article-
Scientific Paper
Oligomeric Aβ in the cerebrospinal fluid of patients with early Alzheimer's Disease: A pilot study.
Kyewords:
Alzheimer's Disease / Amyloidβ / Amyloid Beta / Beta Amyloid / Aβ / Oligomers / Toxic Oligomers / ELISA / Amyloidβ (1-42) / Amyloidβ (1-40) / Diabetes
The control set is available for sale on request. Learn More
Trend of Toxic Amyloidβ Oligomer Hypothesis
Related IBL News
Relationship between mild cognitive impairment (MCI) which suggests early stage of Alzheimer's disease and Amyloid β toxic oligomer –new article-
Scientific Paper
Oligomeric Aβ in the cerebrospinal fluid of patients with early Alzheimer's Disease: A pilot study.
Kyewords:
Alzheimer's Disease / Amyloidβ / Amyloid Beta / Beta Amyloid / Aβ / Oligomers / Toxic Oligomers / ELISA / Amyloidβ (1-42) / Amyloidβ (1-40) / Diabetes
References
References
- Structural basis of the 24B3 antibody against the toxic conformer of amyloid β with a turn at positions 22 and 23. Irie Y et al. Biochem Biophys Res Commun. 2022 Sep 17;621:162-167.PMID: 35839743
- Toxic Amyloid-β42 Conformer May Accelerate the Onset of Alzheimer's Disease in the Preclinical Stage. Futamura A et al. J Alzheimers Dis. 2021 Feb 9.PMID: 33579852
- Control of the Toxic Conformation of Amyloid β42 by Intramolecular Disulfide Bond Formation. Matsushima Y et al. Chem Commun (Camb). 2020 Apr 9;56(29):4118-4121.PMID: 32163091
- New Diagnostic Method for Alzheimer's Disease Based on the Toxic Conformation Theory of Amyloid β. Irie K. Biosci Biotechnol Biochem. 2020 Jan;84(1):1-16.PMID: 31538538
- Insulin deficiency promotes formation of toxic amyloid-β42 conformer co-aggregating with hyper-phosphorylated tau oligomer in an Alzheimer's disease model. Imamura T et al. Neurobiol Dis. 2020 Jan 9:104739.PMID: 31927145
- An App knock-in mouse inducing the formation of a toxic conformer of Aβ as a model for evaluating only oligomer-induced cognitive decline in Alzheimer's disease. Izuo N et al. Biochem Biophys Res Commun. 2019 Jul 30;515(3):462-467.PMID: 31164199
- A Toxic Conformer of Aβ42 with a Turn at 22-23 is a Novel Therapeutic Target for Alzheimer's Disease. Izuo N et al. Sci Rep. 2017 Sep 18;7(1):11811.PMID: 28924167
- Monoclonal antibody with conformational specificity for a toxic conformer of amyloid β42 and its application toward the Alzheimer's disease diagnosis. Murakami K et al. Sci Rep. 2016 Jul 4;6:29038PMID: 27374357
Note: Retrieve by PMID number in displayed by abstract: http://www.ncbi.nlm.nih.gov
FAQ
FAQ
-
Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
ELISA common FAQ -
A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
-
Q.What is the composition of concentrated wash buffer?
ELISA common FAQ -
A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
-
Q.What is the feature of the plate?
ELISA common FAQ -
A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
-
Q.Can I re-use standard after reconstitution?
ELISA common FAQ -
A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
Please check the details on each product datasheet. -
Q.What is different between reagent blank and test sample blank?
ELISA common FAQ -
A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the Test sample value is influenced by lack of washing process or other operations. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
-
Q.How many samples can be measured by this kit?
ELISA common FAQ -
A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
-
Q.What is LOD (Limit of Detection)?
ELISA common FAQ -
A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
-
Q.What is LOQ (Limit of Quantification)?
ELISA common FAQ -
A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
-
Q.What is the definition of Over Night (O/N) reaction?
ELISA common FAQ -
A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.
-
Q.What is the specification of quality control for ELISA product release?
ELISA common FAQ -
A.The information of specification is available on individual lot specific CoA. Please contact us with your reference lot number for obtaining of specific CoA.
-
Q.What is the number (e.g. 432143214321) at the edge of strips of the plate?
ELISA common FAQ -
A.According to the plate maker (ThermoFisher), it does not have any specific meaning as it is just the number of molds.
-
Q.How to wash an ELISA plate?
ELISA common FAQ -
A.Washing it by an auto-washer is highly recommended.
If it is not available, please refer to the demo video (only 2 mins) using a washing bottle. -
Q.The wells turned black during the test with the kit.
ELISA common FAQ -
A.It is possible that the wells were not washed sufficiently during the washing process after the HRP-labeled antibody reaction.
Be sure to wash the wells enough times as described in the data sheet with washing buffer of more than 350 µL. -
Q.How do you define the acceptance range of quality control of control sets?
Control Set Common FAQ -
A.In principal, it is defined as ±15%-20% based the standard value described on each datasheet.
-
Q.What is the shelf life of control set?
Control Set Common FAQ -
A.The shelf life is 12 months upon the goods is dispatched from our lab if the goods is unopned.
-
Q.What is the storage condition of control sets?
Control Set Common FAQ -
A.It should be stored under chilled condition (2~10℃).
-
Q.Can the control set be used after reconstitution?
Control Set Common FAQ -
A.No it cannot be re-used after the reconstitution.
-
Q.What is the package form of control sets?
Control Set Common FAQ -
A.It is lyophilized form.