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#27998 Human soluble α-Klotho Assay Kit - IBL

  • alpha-Klotho comparison data N=10 (same donor)
  • #27998 Human soluble α-Klotho ELISA Kit
  • #27998 Human soluble α-Klotho ELISA Kit
  • #27998 Human soluble α-Klotho ELISA Kit
  • #27998 Human soluble α-Klotho ELISA Kit
  • #27998 Human soluble α-Klotho ELISA Kit
  • #27998 Human soluble α-Klotho ELISA Kit
  • α-Klotho, FGF23 in CKD
  • #27998 Human soluble α-Klotho ELISA Kit
  • #27998 human α- Klotho ELISA Sample Stability Test (freeze-thaw)
  • #27998 human α- Klotho ELISA Sample Stability Test （37℃ Accerelation Test)
  • #27998 human α- Klotho ELISA_Blood Samples Measurement Data IBL
  • #27998 human α- Klotho ELISA_Cross Reaction Test with β-Klotho
Intended Use：
Research reagents
Measuring Method：
ELISA
Sample Types：
Human
Measuring Samples：
Human serum, EDTA Plasma, Heparin Plasma, Citrated Plasma, Urine and cell culture supernatant.
Measurement Range：
93.75 - 6,000 pg/mL
Package Size1：
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
　 for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
　 enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 27998
Product Name Human soluble α-Klotho Assay Kit - IBL
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human
Measuring Samples Human serum, EDTA Plasma, Heparin Plasma, Citrated Plasma, Urine and cell culture supernatant.
Measurement Range 93.75 - 6,000 pg/mL
Primary Reaction 60 minutes at room temperature
Secondary Reaction 30 minutes at room temperature
Sensitivity 6.15 pg/mL
Specificity Compound Cross Reactivity
Human α-Klotho 100 %
Human secreted α-Klotho 100 %
Human β-Klotho ＜0.1 %
Human osteopontin ＜0.1 %
Human VEGF (165) ＜0.1 %
Human PDGF ＜0.1 %
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Available
Package Size 1 96 Well

Product Description

Product Description

α-Klotho (Alpha Klotho) was identified as an extremely down-regulated gene in the genetically-modified mouse of which phenotype is very similar to various symptoms of human aging. The sequences of Alpha Klotho genes have been identified in various species including humans based on the one of mouse. Alpha Klotho protein is a 130 kDa, one-transmembrane protein and its expression is confirmed in the kidneys and the parathyroid glands. In recent years, it has become clear that Alpha Klotho is an important molecule within a living organism regulating the metabolism of mineral such as calcium and phosphorus. It is considered that in Alpha Klotho mouse, early aging-like symptoms are induced by impaired mineral homeostasis caused by decreased expression of Alpha Klotho. Meanwhile, it is reported that the long N-terminal extracellular domain which comprises the major portion of sequence of Alpha Klotho protein is released free into blood by shedding. However, there are many unclear points about functions and changes in concentration of free (soluble) Alpha Klotho protein, so it was required to develop the measurement system of Alpha Klotho. This ELISA kit can measure Human soluble Alpha Klotho protein in human blood.

The control set is available for sale on request. Learn More

Keywords:
α-Klotho / Alpha Klotho / FGF23 / Kidney disease / Chronic Kidoeny disease (CKD) / Mineral Bone Disease (MBD) / Osteoporosis / Aging / Alzheimer's Disease　

References

References

Note: Retrieve by PMID number in displayed by abstract: http://www.ncbi.nlm.nih.gov

FAQ

FAQ

  • Question
    Q.Can this kit measure the full length of α-Klotho?
  • Answer
    A.Yes, it can be used to measure the full length α-Klotho and KL1.
  • Question
    Q.What are the epitopes of pre-coated antibody (Anti-human Klotho (67G3) Mouse IgG MoAb A.P.) and HRP conjugated antibody (Human Klotho (91F1) Mouse IgG MoAb Fab’ A.P.)?
  • Answer
    A.Klotho has two domains (KL1 and KL2). Although we have not conducted epitope mapping, these antibodies can detect KL1 domains.
  • Question
    Q.Do you have any information regarding the specificity of the kit?
  • Answer
    A.Please refer to Fig 1 on page 10 of the following article (PMID:20599764).

    Establishment of sandwich ELISA for soluble alpha-Klotho measurement: Age-dependent change of soluble alpha-Klotho levels in healthy subjects. Yamazaki Y et al. Biochem Biophys Res Commun. 2010 Jul 30;398(3):513-8.
  • Question
    Q.Does this kit cross react with β-Klotho?
  • Answer
    A.No, it does not.　As the data is listed on the product page, please refer to it.
    https://www.ibl-japan.co.jp/en/search/product/detail/id=3959
  • Question
    Q.Can this ELISA detect other species rather than human such as canine (dog), rabbit, rat and mouse?
  • Answer
    A.No it cannot. However, cynomolgus samples is possible to be measured. Mouse urine samples can be measured by #27601 Mouse soluble α-Klotho Assay Kit - IBL.
  • Question
    Q.What kind of plasma sample can be used?
  • Answer
    A.Typical data of the performance of the kit were obtained using human serum, EDTA-plasma, urine and cell culture supernatant for this kit, however, as you can see the data of heparin-plasma and citrated plasma measured by this kit displayed on this product page, they can be also measured by this kit.
  • Question
    Q.Is any sample stability data available?
  • Answer
    A.Yes, it is available on the following relevant product page. Please refer to the Pamphlet listed on the page.
    http://www.ibl-japan.co.jp/en/search/product/detail/id=3959
  • Question
    Q.What is pM unit of standards?
  • Answer
    A.The 6,000pg/mL of standard in this kit corresponds to 55pM (as Molecular Weight 108.99kDa). Please refer to the article Establishment of sandwich ELISA for soluble alpha-Klothomeasurement: Age-dependent change of soluble alpha-Klotho levels in healthy subjects YYamazaki et al. Biochem Biophys Res Commun. 2010 July 30; 398(3): 513–518 (PMID 20599764 / https://www.ncbi.nlm.nih.gov/pubmed/?term=20599764 ) for further information of development of this kit.
  • Question
    Q.What is the difference between soluble and secreted α-Klotho kits?
  • Answer
    A.The α-Klotho protein has been reported to shedding its long N-terminal extracellular domain, which occupies most of the sequence, and to be released in the blood (soluble). At the same time, gene splicing has been reported to produce a secreted isoform with only the first repeat (secreted).
    #27998 Human soluble α-Klotho Assay Kit - IBL specifically detects all soluble α-Klotho including the KL1 domain.
    #27901 Human secreted α-Klotho Assay Kit - IBL specifically detects secreted α-Klotho in the KL1 domain, including the sequence QVSQLTKPISSLTKPYH.
  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the Test sample value is influenced by lack of washing process or other operations. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
  • Question
    Q.What is the definition of Over Night (O/N) reaction?
    ELISA common FAQ
  • Answer
    A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.
  • Question
    Q.What is the specification of quality control for ELISA product release?
    ELISA common FAQ
  • Answer
    A.The information of specification is available on individual lot specific CoA. Please contact us with your reference lot number for obtaining of specific CoA.
  • Question
    Q.What is the number (e.g. 432143214321) at the edge of strips of the plate?
    ELISA common FAQ
  • Answer
    A.According to the plate maker (ThermoFisher), it does not have any specific meaning as it is just the number of molds.
  • Question
    Q.How to wash an ELISA plate?
    ELISA common FAQ
  • Answer
    A.Washing it by an auto-washer is highly recommended.
    If it is not available, please refer to the demo video (only 2 mins) using a washing bottle.
  • Question
    Q.The wells turned black during the test with the kit.
    ELISA common FAQ
  • Answer
    A.It is possible that the wells were not washed sufficiently during the washing process after the HRP-labeled antibody reaction.
    Be sure to wash the wells enough times as described in the data sheet with washing buffer of more than 350 µL.
  • Question
    Q.How do you define the acceptance range of quality control of control sets?
    Control Set Common FAQ
  • Answer
    A.In principal, it is defined as ±15%-20% based the standard value described on each datasheet.
  • Question
    Q.What is the shelf life of control set?
    Control Set Common FAQ
  • Answer
    A.The shelf life is 12 months upon the goods is dispatched from our lab if the goods is unopned.
  • Question
    Q.What is the storage condition of control sets?
    Control Set Common FAQ
  • Answer
    A.It should be stored under chilled condition (2～10℃).
  • Question
    Q.Can the control set be used after reconstitution?
    Control Set Common FAQ
  • Answer
    A.No it cannot be re-used after the reconstitution.
  • Question
    Q.What is the package form of control sets?
    Control Set Common FAQ
  • Answer
    A.It is lyophilized form.
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