#10387 Anti- Smad3L (S213 Phosphorylated) (5A11) Mouse IgG MoAb

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Intended Use：: Research reagents

Application：: WB, IP, IHC

Package Size1：: 50 μg

Package Size2：: 5 μg

Note on Application Abbreviations: WB:Western Blotting; IP:Immunoprecipitation; IHC:Immunohistochemistry

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
　 for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
　 enclosed in the product purchased before use.

Product Overview

Product Code	10387
Product Name	Anti- Smad3L (S213 Phosphorylated) (5A11) Mouse IgG MoAb
Intended Use	Research reagents
Application	WB, IP, IHC
Species	Human
Immunizing antigen	Synthetic peptide of phosphorylated Smad3L (S213)
Source	Mouse-Mouse hybridoma (X63 - Ag 8.653 × BALB/c mouse spleen cells)
Clone Name	5A11
Subclass	IgG1
Purification Method	Affinity purified with antigen peptide
Specificity	Reacts with phosphorylated Smad3L (Ser 213) of human in specific.
Package Form	Lyophilized product in PBS containing 1 % BSA and 0.05 % NaN3
Storage Condition	2 - 8℃
Poisonous and Deleterious Substances	Applicable
Cartagena	Not Applicable
Package Size 1	50 μg
Package Size 2	5 μg
Remarks1	The commercial use of products without our permission is prohibited. Please make sure to contact us and obtain permission.

Product Description

Phosphorylation of signal transduction molecules Smad3, can be an important information for understanding of various biological functions of transforming growth factor (TGF)-β. TGF-β type I receptor and mitogen activated protein kinase (MAPK) phosphorylate Smad3 at the C-terminals and at linker (middle) regions respectively. Mitogenic signaling induced by HGF, EGF and inflammatory cytokines is mediated by Smad3 phosphoisoform phophorylated serine located in linker region. This monoclonal antibody against for Smad3L (S213) recognizes the part of phosphorylated serine (Ser213) located in linker region of Smad3 specifically. It can be used for western blotting, immunoprecipitation and immunohistochemistry. It is applicable for enzyme biochemical analysis of Smad3 signaling and makes it possible to visualize the intermolecular reaction of phosphorylated Smad3 signaling in human tissues and monitor them in real-time. Thus, analysis of Smad3 signaling with this antibody is expected to be widely applied to cancer serearch and fibrosis research, and to contribute to understanding the wide variety of life phenomina mediated by phosphoisoforms of Smad3.