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#10031 Anti-Tob (4B1) Mouse IgG MoAb

  • WB
  • Human esophagus cancer
Intended Use：
Research reagents
Application：
WB, IHC
Package Size1：
200 μg
Package Size2：
10 μg
Note on Application Abbreviations
WB:Western Blotting
IHC:Immunohistochemistry

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
　 for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
　 enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 10031
Product Name Anti-Tob (4B1) Mouse IgG MoAb
Intended Use Research reagents
Application WB, IHC
Species Human
Immunizing antigen Recombinant Tob
Source Mouse-Mouse hybridoma(X63 - Ag 8.653 × BALB/c mouse spleen cells, supernatant)
Clone Name 4B1
Subclass IgG2a
Purification Method Affinity purified with protein A
Package Form Lyophilized product from 1 % BSA in PBS containing 0.05 % NaN3
Storage Condition 2 - 8℃
Poisonous and Deleterious Substances Applicable
Cartagena Not Applicable
Package Size 1 200 μg
Package Size 2 10 μg
Remarks1 The commercial use of products without our permission is prohibited. Please make sure to contact us and obtain permission.

Product Description

Product Description

Tob (Transducing molecule of c-ErbB-2) was identified as a molecule that binds to
the receptor tyrosine kinase c-ErbB2 in 1996.
After that, Tob2, BTG1, PC3/TIS21/BTG2, ANA, PC3B etc were found as proteins with a homologous region at about 110 amino acids on the N-terminal side, and are called Tob family proteins.
Tob family proteins are known to suppress cell proliferation when compulsorily expressed in cultured cells.
This proliferative suppression occurs by blocking the expression of cyclin D1.
On the other hand, Tob is rapidly phosphorylated at Ser152, Ser154 and Ser164 by Erk1/2 upon growth-factor stimulation.
It is suggested that this phosphorylation cancels the suppression of cyclin D1 expression.
Thus, it is to thought be Tob and its phosphorylation have important role in the progression from G0 to G1 in the cell cycle.

FAQ

FAQ

  • Question
    Q.Which Tob is detected by this antibody?
  • Answer
    A.The antibody both phosphorylated and non-phosphorylated Tob1 detects.
  • Question
    Q.What is the molecular size of Tob1?
  • Answer
    A.As the amino acid sequence of Tob1 is 345 a.a, it is approx. 38kDa based on the calculation.
    The molecular size of phosphorylated Tob1 is bigger than non-phosphorylated and the size is suggested as around 45kDa - 50kDa.
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