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ELISA - IBL（）

27182
Human EL Full-Length Assay Kit - IBL
  • Question
    Q.How can I store the standard after reconstitute it?
  • Answer
    A.It cannot be recommended to be stored after reconstitution as it is designed for use one-off. However, if you need to reuse it due to uncontrolable situation, please store it in frozen.
27725
Human Amyloidβ Oligomers (82E1-specific) Assay Kit - IBL
  • Question
    Q.What size of oligomer can be detected by this assay kit?
  • Answer
    A.Any oligomer that is bigger than Amyloidβ dimer and has a recognized region of 82E1 (N-terminal of 1-16) can be detected by this assay kit.
  • Question
    Q.Can this kit detect protofibril and fibril?
  • Answer
    A.It cross reacts with both protofibril and fibril.
  • Question
    Q.3 recognized parts of 82E1 antibody (a.a. 1-16) are existed if the sample is trimer. In that case, does the conjugated antibody binds to 2parts or only one part?
  • Answer
    A.82E1 antibody is used for as capture antibody (precoated one) and detection antibody (HRP conjugated one). Theoretically, 2 regions of trimer can bind to the detection antibody if 1 region is captured by captured antibody. However, this is only the case that actual molecule detected holds the N-terminal region of Aβ1-16.
  • Question
    Q.Is it depending on the size of oligomer to determine the quantity of the detection antibody that binds to one oligomer?
  • Answer
    A.It depends on molecule of oligomer. It does not detect monomer. We recommend to use both products #27718 Human Amyloidβ (1-40) (FL) Assay Kit - IBL and #27719 Human Amyloidβ (1-42) (FL) Assay Kit - IBL by depending on purposes.
  • Question
    Q.What is recommended dilution ratio for serum samples?
  • Answer
    A.It is 2 to 8 fold.
27181
Human ApoB-100 Assay Kit - IBL
  • Question
    Q.Can you verify that it is HIV or HBV negative?
  • Answer
    A.The plasma used as the material was verified as negative and not cross reactive against HbsAG, anti-HCV, anti-HBc by the method of approved by FDA.
  • Question
    Q.What is used for the standard?
  • Answer
    A.It is ApoB 100 that is purified from human plasma.
27102
Mouse VEGF Assay Kit - IBL
  • Question
    Q.Does this assay kit detect any specific type of VEGF or any type of VEGF such as A, B, C and D?
  • Answer
    A.It cross reacts with VEGF-A. Isoforms like 120, 164 and 188 can be measured as mixed substances if the sample is lysate.
  • Question
    Q.Is it possible to measure VEGF expressed in lysate of mice muscle?
  • Answer
    A.If it is an ordinary tissue lysate, it can be measured by the assay kit.
27785
Mouse LRG Assay Kit - IBL
  • Question
    Q.What is the structure and host of the standard?
  • Answer
    A.It is originated from CHO supernatant and it is full length Flag-Tag.
27194
Rat TNF-α Assay Kit - IBL
  • Question
    Q.Can this kit be used for serum?
  • Answer
    A.No it cannot be used for normal serum.
27169
Endothelin-3 Assay Kit - IBL (Product On Request)
  • Question
    Q.Why extraction and condensation process using Sep-Pack is required for measuring Endothelin-3 (ET-3) blood samples?
    Is the process essential for the assay?
  • Answer
    A.Measuring ET-3 in blood sample is quite difficult due to very low concentration of ET-3 (reference approx. 0.48pg/mL in normal subject blood sample).
    The extraction and condensation process using Sep-Pack is essential for the assay.
    The assay will be failed without the process.
    The ET-3 in blood sample become much lower than the original concentration after the process and it depends on the collection rate.
    Therefore, it is recommended to prepare larger volume 5mL-10mL of blood sample for ET-3 measurement.
    Please refer to "Attention for sample handling" on page 2 of the datasheet for additional information.
  • Question
    Q.Which region are recognized by the antibodies used by this kit?
  • Answer
    A.The capture antibody (Anti-ET 15-21) recognizes the region at C-terminal of ET-3 and the detection antibody specifically recognizes the region at N-terminal of ET-3.
  • Question
    Q.How should I conduct Endothelin extraction process?
  • Answer
    A.Please refer to the datasheet enclosed the product package and/or the datasheet listed on the product page.
    As several publications can be found on the page (product description), please refer to them.
    https://www.ibl-japan.co.jp/en/search/product/detail/id=3869
  • Question
    Q.Is extraction process essential for blood samples?
  • Answer
    A.Yes, it is essential. Please follow the datasheet for the extraction process using column such as SepPak.
  • Question
    Q.I have only 0.5mL blood samples. Is it possible to measure it using this ELISA?
  • Answer
    A.Yes, it is possible, however, in general speaking, the concentration of Endothelin in blood sample is low conc., the concentration could be much lower after the extraction and process. Please well consider the risk of lack of concentration prior start the measurement.
    Our recommendation is more than 2mL blood samples.
27998
Human soluble α-Klotho Assay Kit - IBL
  • Question
    Q.Can this kit measure the full length of α-Klotho?
  • Answer
    A.Yes, it can be used to measure the full length α-Klotho and KL1.
  • Question
    Q.What are the epitopes of pre-coated antibody (Anti-human Klotho (67G3) Mouse IgG MoAb A.P.) and HRP conjugated antibody (Human Klotho (91F1) Mouse IgG MoAb Fab’ A.P.)?
  • Answer
    A.Klotho has two domains (KL1 and KL2). Although we have not conducted epitope mapping, these antibodies can detect KL1 domains.
  • Question
    Q.Do you have any information regarding the specificity of the kit?
  • Answer
    A.Please refer to Fig 1 on page 10 of the following article (PMID:20599764).

    Establishment of sandwich ELISA for soluble alpha-Klotho measurement: Age-dependent change of soluble alpha-Klotho levels in healthy subjects. Yamazaki Y et al. Biochem Biophys Res Commun. 2010 Jul 30;398(3):513-8.
  • Question
    Q.Does this kit cross react with β-Klotho?
  • Answer
    A.No, it does not.　As the data is listed on the product page, please refer to it.
    https://www.ibl-japan.co.jp/en/search/product/detail/id=3959
  • Question
    Q.Can this ELISA detect other species rather than human such as canine (dog), rabbit, rat and mouse?
  • Answer
    A.No it cannot. However, cynomolgus samples is possible to be measured. Mouse urine samples can be measured by #27601 Mouse soluble α-Klotho Assay Kit - IBL.
  • Question
    Q.What kind of plasma sample can be used?
  • Answer
    A.Typical data of the performance of the kit were obtained using human serum, EDTA-plasma, urine and cell culture supernatant for this kit, however, as you can see the data of heparin-plasma and citrated plasma measured by this kit displayed on this product page, they can be also measured by this kit.
  • Question
    Q.Is any sample stability data available?
  • Answer
    A.Yes, it is available on the following relevant product page. Please refer to the Pamphlet listed on the page.
    http://www.ibl-japan.co.jp/en/search/product/detail/id=3959
  • Question
    Q.What is pM unit of standards?
  • Answer
    A.The 6,000pg/mL of standard in this kit corresponds to 55pM (as Molecular Weight 108.99kDa). Please refer to the article Establishment of sandwich ELISA for soluble alpha-Klothomeasurement: Age-dependent change of soluble alpha-Klotho levels in healthy subjects YYamazaki et al. Biochem Biophys Res Commun. 2010 July 30; 398(3): 513–518 (PMID 20599764 / https://www.ncbi.nlm.nih.gov/pubmed/?term=20599764 ) for further information of development of this kit.
  • Question
    Q.What is the difference between soluble and secreted α-Klotho kits?
  • Answer
    A.The α-Klotho protein has been reported to shedding its long N-terminal extracellular domain, which occupies most of the sequence, and to be released in the blood (soluble). At the same time, gene splicing has been reported to produce a secreted isoform with only the first repeat (secreted).
    #27998 Human soluble α-Klotho Assay Kit - IBL specifically detects all soluble α-Klotho including the KL1 domain.
    #27901 Human secreted α-Klotho Assay Kit - IBL specifically detects secreted α-Klotho in the KL1 domain, including the sequence QVSQLTKPISSLTKPYH.
27131
Human G-CSF Assay Kit - IBL
  • Question
    Q.Can this kit be used for mouse or rat samples?
  • Answer
    A.No, they cannot.
  • Question
    Q.What is the epitope of antibodies used in this ELISA?
  • Answer
    A.Since a whole recombinant human G-CSF was used as the immunogen for these antibodies and no epitope mapping has been done, the information is not available.
27998
Human soluble α-Klotho Assay Kit - IBL
  • Question
    Q.Can this kit detect enzymatic activity?
  • Answer
    A.No it cannot.
  • Question
    Q.Can this kit use for cow, mouse or rat samples?
  • Answer
    A.No, it cannot.
27732
Human sAPPβ-w (highly sensitive) Assay Kit - IBL
  • Question
    Q.What is epitope of the antibodies used in this kit?
  • Answer
    A.The epitope of the HRP conjugated labeled antibody (Anti-Human APP (R101A4) Mouse IgG Fab’ Affinity Purify) used in this kit has not been confirmed.

    The epitope of the precoated plate antibody (Anti-Human sAPPβ-wild Type Rabbit IgG Affinity Purify at C-Terminal) used in this kit is ISEVKM.
  • Question
    Q.What type of soluble APP can be measured by this kit?
  • Answer
    A.This kit specifically detects human soluble APPβ wild type (sAPPβ-w).
  • Question
    Q.What the standards are derived from?
  • Answer
    A.It is recombinant protein originated from CHO.
  • Question
    Q.What is the optimal dilution ratio for CSF samples measurement?
  • Answer
    A.Please refer to the section "PERFORMANCE CHARACTERISTICS" on page 2 of the datasheet.
    2-4 fold dilution is recommended to start for finding the optimal dilution ratio.
    https://www.ibl-japan.co.jp/files/topics/1668_ext_02_en_0.pdf
  • Question
    Q.What is the optimal dilution ratio for EDTA-plasma samples measurement?
  • Answer
    A.Please refer to the section "PERFORMANCE CHARACTERISTICS" on page 2 of the datasheet.
    2-4 fold dilution is recommended to start for finding the optimal dilution ratio.
    https://www.ibl-japan.co.jp/files/topics/1668_ext_02_en_0.pdf
  • Question
    Q.Can this kit measure sAPPβ in serum samples?
  • Answer
    A.No, it is not recommended. sAPPβ in serum cannot be measured by this kit. Please use EDTA-plasma samples for the kit.
17165
Endothelin-1 Assay Kit - IBL
  • Question
    Q.Can this kit be used for heparin-plasma samples?
  • Answer
    A.Endothelin-1 in heparin plasma can be measured. However, the extraction process must be performed. For details about the extraction process, see “12. Endothelin extraction process” on page 2 of the datasheet. Please refer to the datasheet for additional information.
  • Question
    Q.What kind of animal samples can be used with this kit?
  • Answer
    A.Human, rat, pig, canine, mouse, cow, rabbit samples can be used with the kit as epitope of No.1 to 21 of Endothelin-1 is same among the species.
  • Question
    Q.In regards to the extraction of the plasma sample, can I use a column other than C18?
  • Answer
    A.Please ask the maker for the column you intend to use.
  • Question
    Q.What kind of Sep-pak should be used for the assay?
  • Answer
    A.We use Sep-pak light.
  • Question
    Q.Is a correlation data with #27165 available?
  • Answer
    A.Yes, it is. The data is listed on the product detail page as a photo image. Please refer to the data.
  • Question
    Q.Can I use "reconstituted labeled antibody" after re-stored at -20℃?
  • Answer
    A.Not recommended. Please use labeled antibody immediately after the reconstitution.
  • Question
    Q.How should I conduct Endothelin extraction process?
  • Answer
    A.Please refer to the datasheet enclosed the product package and/or the datasheet listed on the product page.
    As several publications can be found on the page (product description), please refer to them.
    https://www.ibl-japan.co.jp/en/search/product/detail/id=3866
  • Question
    Q.Is extraction process essential for blood samples?
  • Answer
    A.Yes, it is essential. Please follow the datasheet for the extraction process using column such as SepPak.
  • Question
    Q.I have only 0.5mL blood samples. Is it possible to measure it using this ELISA?
  • Answer
    A.Yes, it is possible, however, in general speaking, the concentration of Endothelin in blood sample is low conc., the concentration could be much lower after the extraction and process. Please well consider the risk of lack of concentration prior start the measurement.
    Our recommendation is more than 2mL blood samples.
27258
Human Osteopontin N-Half Assay Kit - IBL
  • Question
    Q.Can this kit be used for crab-eating macaque samples?
  • Answer
    A.We do not have any data related to those types of samples.
  • Question
    Q.Can this kit be used for rat samples?
  • Answer
    A.No, it cannot.
  • Question
    Q.Can this kit be used for bovine samples?
  • Answer
    A.No, it cannot.
  • Question
    Q.What is the epitope of the capture antibody Anti- Osteopontin N-Half(34E3) Mouse IgG MoAb.?
  • Answer
    A.It is SVVYGLR where is the position of thrombin cleavage site of human OPN so that this antibody specifically recognize the position at OPN N-Half.
  • Question
    Q.What kind of Osteopontin isoforms can be measured by this kit?
  • Answer
    A.This kit can specifically measure human OPN N-Half that cleaved by thrombin.
27158
Human Osteopontin Assay Kit - IBL
  • Question
    Q.Can this kit be used for serum samples?
  • Answer
    A.Osteopontin is rapidly digested by Thrombin in serum samples. For this reason, we do not recommend using serum samples for the assay because the value would be much lower.
  • Question
    Q.There are two types of EDTA-plasma tubes (Na and K). Which one is best to use?
  • Answer
    A.Either tube can be used.
  • Question
    Q.What is the epitope of the pre-coated antibody (Anti-Human OPN (O-17) Rabbit IgG A.P.)?
  • Answer
    A.The epitope is IPVKQADSGSSEEKQ.
  • Question
    Q.Can this kit be used to measure crab-eating macaque samples?
  • Answer
    A.We do not have any data related to those types of samples.
  • Question
    Q.Can this kit be used for citrated-plasma samples?
  • Answer
    A.We do not recommend using the kit for citrated-plasma samples.
  • Question
    Q.Can this kit be used for bovine samples?
  • Answer
    A.No, it cannot.
  • Question
    Q.Is any sample stability data available?
  • Answer
    A.Yes, it is available on the following relevant product page.
    Please refer to the Pamphlet 2 listed on the page.
    http://www.ibl-japan.co.jp/en/search/product/detail/id=3862
  • Question
    Q.What is the epitope of the detection antibody Anti-Human OPN（10A16）Mouse IgG MoAb Fab’A.P.?
  • Answer
    A.It is KSKKFRRPDIQYPDATDE where is at the part of the right side position of thrombin cleavage site of human Osteopontin (OPN).
  • Question
    Q.What kind of Osteopontin isoforms can be measured by this kit?
  • Answer
    A.This kit uses the antibody (#18625 OPN (O-17)) that recognizes the region at N-terminal of Osteopontin (OPN) as the capture antibody and and the antibody (#10011 OPN(10A16)) that recognizes the region at the part of the right side position of thrombin cleavage site of human OPN as the detection antibody. Therefore, this kit can detect all human OPN contains N-term region and the region described (10A16) above including Full Length of OPN. However, OPN cleaved by Thrombin cannot be measured by this kit. Please use #27258 Human Osteopontin N-Half Assay Kit - IBL for measurement of human OPN N-Half.
27171
Human VEGF Assay Kit - IBL
  • Question
    Q.Is there any cross reactivity other than VEGF161?
  • Answer
    A.The specificity is described in “10. Kit performance” on the datasheet. Please refer to the datasheet for additional information.
  • Question
    Q.Can this kit be used for plasma samples?
  • Answer
    A.EDTA-plasma can be used. However, heparin-plasma cannot be used for the assay.
  • Question
    Q.What is recombinant VEGF derived from?
  • Answer
    A.It is a supernatant of CHO.
  • Question
    Q.Can this kit be used for mice and rat samples?
  • Answer
    A.No, it cannot be used for those animal samples.
27769
Human LRG Assay Kit - IBL (Discontinued : until the raw materials are ended)
  • Question
    Q.What is the expression system of the standard?
  • Answer
    A.It is a recombinant protein expression system. Wheat germ is used in this system.
  • Question
    Q.What is the recommended dilution ratio?
  • Answer
    A.We cannot recommend a specific dilution ratio, but we generally use a dilution magnification of anywhere between 2 to 16 when we conduct measurements.
  • Question
    Q.What is the epitopes of the pre-coated antibodies used in this kit?
  • Answer
    A.The epitope of the anntibody (Human LRG (162) Rabbit IgG A.P.) is GLKALGHLSGNRLRKL.
  • Question
    Q.What is the epitope of the labeled antibody?
  • Answer
    A.The epitope of the labeled antibody (Human LRG (329) Rabbit IgG Fab’ A.P.) is AGPEAVKGQTLLAVAKSQ.
  • Question
    Q.Is there any data available for the measurement example and the reproducibility using normal controls?
  • Answer
    A.Yes there is. The date is available on the following relevant product page. Please refer to the Pamphlet 2 listed on the page.
    http://www.ibl-japan.co.jp/en/search/product/detail/id=3945
  • Question
    Q.Does this kit specifically detect LRG1 or other LRG families as well?
  • Answer
    A.We have not actually checked that the cross reactivity against other LRG families using our LRG-1 kit #27769, however, as epitope of the antibodies that are used for ELISA kit are different positions from Leucine-rich repeat region, theoretically we believe that it specifically detects LRG1 and does not cross react to other LRG families.
27719
Human Amyloidβ (1-42) (FL) Assay Kit - IBL
  • Question
    Q.Can this kit be used for serum samples?
  • Answer
    A.We do not recommend using serum samples for the kit.
  • Question
    Q.Do you have any protocol for preparation of mice and/or rat brain
    tissue extracts?
  • Answer
    A.CHAPS is recommended for extractin of wild type (W.T.) mice or rat brain tissue.
    Please refer to the following link for the brain tissue extraction process using CHAPS.
    http://www.ibl-japan.co.jp/files/topics/4944_ext_02_0.pdf

    Guanidine solution is recommended for extraction of Transgenic (Tg) mice or rat brain tissue.
    Please refer to the following link and the following two scientific articles for brain tissue extraction process using Guanidine.
    http://www.ibl-japan.co.jp/files/topics/4945_ext_02_0.pdf

    Presenilin-2 mutation causes early amyloid accumulation and memory impairment in a transgenic mouse model of Alzheimer's disease. Toda T et al. J Biomed Biotechnol. 2011;2011:617974.
    https://www.ncbi.nlm.nih.gov/pubmed/?term=21234330

    Methylene blue modulates β-secretase, reverses cerebral amyloidosis, and improves cognition in transgenic mice. Mori T et al. J Biol Chem. 2014 Oct 31;289(44):30303-17.
    https://www.ncbi.nlm.nih.gov/pubmed/?term=25157105

    * The ELISA kit #27712 Aβ42 and #27714 Aβ40 have been upgraded to #27719 Aβ (1-42)(FL) and #27718 Aβ (1-40)(FL).
27711
Human Amyloidβ (1-42) Assay Kit - IBL
  • Question
    Q.Can this kit be used for the brain extracts from transgenic mice with Alzheimer's disease?
  • Answer
    A.yes, it can.
  • Question
    Q.Can this kit be used for monkey samples?
  • Answer
    A.Because the epitopes of monkeys and human beings are the same, we believe the kit can also be used for monkey samples. However, we do not have any data to back this claim.
  • Question
    Q.Do you have any protocol for preparation of mice and/or rat brain
    tissue extracts?
  • Answer
    A.CHAPS is recommended for extractin of wild type (W.T.) mice or rat brain tissue.
    Please refer to the following link for the brain tissue extraction process using CHAPS.
    http://www.ibl-japan.co.jp/files/topics/4944_ext_02_0.pdf

    Guanidine solution is recommended for extraction of Transgenic (Tg) mice or rat brain tissue.
    Please refer to the following link and the following two scientific articles for brain tissue extraction process using Guanidine.
    http://www.ibl-japan.co.jp/files/topics/4945_ext_02_0.pdf

    Presenilin-2 mutation causes early amyloid accumulation and memory impairment in a transgenic mouse model of Alzheimer's disease. Toda T et al. J Biomed Biotechnol. 2011;2011:617974.
    https://www.ncbi.nlm.nih.gov/pubmed/?term=21234330

    Methylene blue modulates β-secretase, reverses cerebral amyloidosis, and improves cognition in transgenic mice. Mori T et al. J Biol Chem. 2014 Oct 31;289(44):30303-17.
    https://www.ncbi.nlm.nih.gov/pubmed/?term=25157105

    * The ELISA kit #27712 Aβ42 and #27714 Aβ40 have been upgraded to #27719 Aβ (1-42)(FL) and #27718 Aβ (1-40)(FL).
27713
Human Amyloidβ (1-40) Assay Kit - IBL
  • Question
    Q.Can this kit be used for brain extracts of Alzheimer's disease transgenic mice?
  • Answer
    A.yes, it can.
  • Question
    Q.Can this kit be used for monkey samples?
  • Answer
    A.Because the epitopes of monkeys and human beings are the same, we believe the kit can also be used for monkey samples. However, we do not have any data to back this claim.
  • Question
    Q.Is any special treatment required for serum samples?
  • Answer
    A.No, no special treatment is required.
  • Question
    Q.Do you have any protocol for preparation of mice and/or rat brain
    tissue extracts?
  • Answer
    A.CHAPS is recommended for extractin of wild type (W.T.) mice or rat brain tissue.
    Please refer to the following link for the brain tissue extraction process using CHAPS.
    http://www.ibl-japan.co.jp/files/topics/4944_ext_02_0.pdf

    Guanidine solution is recommended for extraction of Transgenic (Tg) mice or rat brain tissue.
    Please refer to the following link and the following two scientific articles for brain tissue extraction process using Guanidine.
    http://www.ibl-japan.co.jp/files/topics/4945_ext_02_0.pdf

    Presenilin-2 mutation causes early amyloid accumulation and memory impairment in a transgenic mouse model of Alzheimer's disease. Toda T et al. J Biomed Biotechnol. 2011;2011:617974.
    https://www.ncbi.nlm.nih.gov/pubmed/?term=21234330

    Methylene blue modulates β-secretase, reverses cerebral amyloidosis, and improves cognition in transgenic mice. Mori T et al. J Biol Chem. 2014 Oct 31;289(44):30303-17.
    https://www.ncbi.nlm.nih.gov/pubmed/?term=25157105

    * The ELISA kit #27712 Aβ42 and #27714 Aβ40 have been upgraded to #27719 Aβ (1-42)(FL) and #27718 Aβ (1-40)(FL).
27188
Human Syndecan-4 Assay Kit - IBL
  • Question
    Q.Can this kit be used for mouse or rat samples?
  • Answer
    A.No, it cannot.
  • Question
    Q.What is the epitope of antibodies used in this kit?
  • Answer
    A.Anti-Human Syndecan-4 Rabbit IgG (Precoated Antibody) recognizes position no. 45-57 amino acid of human Syndecan-4.
    Anti-Human Syndecan-4 Rabbit IgG (HRP Antibody) recognizes position no. 19-145 amino acid of human Syndecan-4.
  • Question
    Q.What is the immunogen of 2 antibodies used in the kit?
  • Answer
    A.Synthesized Peptide was used as the immunogen for Anti-Human Syndecan-4 Rabbit IgG (Precoated Antibody).
    Recombinant Protein was used as the immunogen for Anti-Human Syndecan-4 Rabbit IgG (HRP Antibody).
27782
soluble (Pro) renin Receptor Assay Kit - IBL
  • Question
    Q.What is the recommended dilution ratio for plasma samples?
  • Answer
    A.We recommend 40 fold dilution.
  • Question
    Q.What is recommended dilution ratio for human blood samples?
  • Answer
    A.It is 40 or more than 40 fold.
  • Question
    Q.Where the epitope (recognizing region) of antibodies used in this kit are?
  • Answer
    A.The recognizing region of the precoated polyclonal antibody (Anti- (Pro)renin receptor Rabbit IgG Affinity Purify) is located at the amino acid numbers of 148 to 165 in the sequence of (pro)renin receptor.
    The recognizing region of the HRP labeled monoclonal antibody (Anti- (P)RR (93A1B) Mouse IgG Fab’ Affinity Purify) is located at the amino acid numbers of 17 to 88 in the sequence of (pro)renin receptor.
27419
Mouse/Rat sAPPα (highly sensitive) Assay Kit - IBL
  • Question
    Q.What is the epitope of the pre-coated antibody (Anti-Mouse APP(599) Rabbit IgG)?
  • Answer
    A.The epitope is DAEFGHDSGFEVRHQK.
  • Question
    Q.Does this kit detect sAPP695α, sAPP751α and sAPP770α?
  • Answer
    A.Yes it does.
    As this kit is a sandwich ELISA that uses antibodies at N-Terminal and C-Terminal of sAPPα, it detects total sAPPα inclusive sAPP695α, sAPP751α and sAPP770α.
27360
Rat Osteopontin Assay Kit - IBL
  • Question
    Q.Can this kit be used for supernatant samples that contain FBS?
  • Answer
    A.Yes it can.
  • Question
    Q.Can this kit be used for cleaved type of Osteopontin?
  • Answer
    A.No it cannot.
  • Question
    Q.Can this kit be used for serum samples?
  • Answer
    A.No, it cannot.
  • Question
    Q.Can this kit be used for heparin-plasma samples?
  • Answer
    A.We do not recommend using heparin plasma with the kit. The use of this plasma can affect the measurement results.
  • Question
    Q.Can this kit be used for bovine samples?
  • Answer
    A.No, it cannot.
27736
Human APP770 Assay Kit - IBL
  • Question
    Q.What is the epitope of the pre-coated antibody (anti-human APP OX2 (351) Rabbit IgG A.P.)?
  • Answer
    A.The epitope is not available because the rights belong to a third party.
  • Question
    Q.Can this kit be used for mouse or rat samples?
  • Answer
    A.No, it cannot.
27412
Human Total Angiotensinogen Assay Kit - IBL
  • Question
    Q.What are the epitopes of the antibodies used in this kit?
  • Answer
    A.The epitope of the pre-coated antibody (Anti-Human AGT (72) Rabbit IgG Affinity Purify) is IQAKTSPVDEKALQDQLVLV. We do not have the epitope for HRP conjugated Anti- Human AGT (601) Mouse IgG, but the detection part is around C-terminal.
  • Question
    Q.What is the best time for collecting urine?
  • Answer
    A.We recommend collecting urine on an as needed basis.
  • Question
    Q.How should urine samples be stored after they are collected?
  • Answer
    A.They should be stored at -80°C immediately after they are collected.
  • Question
    Q.How many minutes should the urine samples be centrifuged, and at what rpm rate?
  • Answer
    A.This process is not required.
  • Question
    Q.Do you have any data of normal value of total Angiotentinogen in human blood and standard range?
  • Answer
    A.Please refer to the following article. https://www.ncbi.nlm.nih.gov/pubmed/?term=17553939
  • Question
    Q.Can dog sample be used for this kit?
  • Answer
    A.The epitope of human and dog are quite different. Therefore, the kit cannot be used for dog sample.