Product related FAQ

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ELISA - IBL（）

27799
Human Uromodulin ELISA Kit - IBL
  • Question
    Q.Can it be measured in urine sample?
  • Answer
    A.Yes, it is measurable, but it is unstable.
    The dilution ratio should be evaluated and determined by each laboratory, but it is considered as it might be in the range between 10,000 and 20,000 fold. In this case, the commercially available product specific dilution buffer (Code: 27799D-30mL) is additionally required.
    uired.
27769
Human LRG Assay Kit - IBL (Discontinued : until the raw materials are ended)
  • Question
    Q.What is the expression of the standard?
  • Answer
    A.It is a recombinant protein expression, which used CHO cells.
  • Question
    Q.What is the recommended dilution factor for serum?
  • Answer
    A.The suitable dilution factor may vary by the condition, but we generally use around x5,000 for the measurements.
  • Question
    Q.What are the epitopes of the plate-coated antibody used in this kit?
  • Answer
    A.Antibody is from recombinant protein antigen. No epitope mapping has conducted.
  • Question
    Q.What is the epitope of the labeled antibody?
  • Answer
    A.Antibody is from recombinant protein antigen. No epitope mapping has conducted.
  • Question
    Q.Are normal controls measurement data and reproducibility data available?
  • Answer
    A.Yes, we have. The date is available on the following product page. Please refer to the Pamphlet 2 listed on the page.
    http://www.ibl-japan.co.jp/en/search/product/detail/id=5671
  • Question
    Q.Does this kit specifically detect LRG1 or other LRG families as well?
  • Answer
    A.We have not checked the cross reactivity against other LRG families using our LRG-1 kit (#27798).
27364
Human T-Cadherin(130K) ELISA Kit - IBL
  • Question
    Q.Does this kit cross react with mouse?
  • Answer
    A.Yes, it does.
27365
Human T-Cadherin(30K,130K) ELISA Kit - IBL
  • Question
    Q.Does this kit cross react with mouse?
  • Answer
    A.No, it does not.
27366
Human T-Cadherin(100K,130K) ELISA Kit - IBL
  • Question
    Q.Does this kit cross react with mouse?
  • Answer
    A.Yes, it does.
27901
Human secreted α-Klotho Assay Kit - IBL
  • Question
    Q.Can mouse secreted α-Klotho be measured by this ELISA kit?
  • Answer
    A.No, it cannot.
  • Question
    Q.Can secreted α-Klotho in supernatant can be detected by this kit?
  • Answer
    A.Yes, it can be detected.
  • Question
    Q.What is the difference between soluble and secreted α-Klotho kits?
  • Answer
    A.The α-Klotho protein has been reported to shedding its long N-terminal extracellular domain, which occupies most of the sequence, and to be released in the blood (soluble). At the same time, gene splicing has been reported to produce a secreted isoform with only the first repeat (secreted).
    #27998 Human soluble α-Klotho Assay Kit - IBL specifically detects all soluble α-Klotho including the KL1 domain.
    #27901 Human secreted α-Klotho Assay Kit - IBL specifically detects secreted α-Klotho in the KL1 domain, including the sequence QVSQLTKPISSLTKPYH.
27191
Human ApoA5 Assay Kit - IBL
  • Question
    Q.What is the immunogen used for the antibodies?
  • Answer
    A.It is full length recombinant ApoA5.
27175
Human TPO Assay Kit - IBL (Product On Request)
  • Question
    Q.What is the origin of the standard enclosed in the kit?
  • Answer
    A.It is purified recombinant protein derived from CHO.
    It does not contain human-derived substances (blood, tissue, etc.).
99999
99999-1 Control Set - IBL common FAQ
  • Question
    Q.How do you define the acceptance range of quality control of control sets?
    Control Set Common FAQ
  • Answer
    A.In principal, it is defined as ±15%-20% based the standard value described on each datasheet.
  • Question
    Q.What is the shelf life of control set?
    Control Set Common FAQ
  • Answer
    A.The shelf life is 12 months upon the goods is dispatched from our lab if the goods is unopned.
  • Question
    Q.What is the storage condition of control sets?
    Control Set Common FAQ
  • Answer
    A.It should be stored under chilled condition (2～10℃).
  • Question
    Q.Can the control set be used after reconstitution?
    Control Set Common FAQ
  • Answer
    A.No it cannot be re-used after the reconstitution.
  • Question
    Q.What is the package form of control sets?
    Control Set Common FAQ
  • Answer
    A.It is lyophilized form.
27167
Rat Big Endothelin-1 Assay Kit - IBL
  • Question
    Q.How should I conduct Endothelin extraction process?
  • Answer
    A.Please refer to datasheet enclosed the product package and/or the datasheet listed on the product page.
    As several publications can be found on the page (product description), please refer to them.
  • Question
    Q.Is extraction process essential for blood samples?
  • Answer
    A.Yes, it is essential. Please follow the datasheet for the extraction process using column such as SepPak.
  • Question
    Q.I have only 0.5mL blood samples. Is it possible to measure it using this ELISA?
  • Answer
    A.Yes, it is possible, however, in general speaking, the concentration of Endothelin in blood sample is low conc., the concentration could be much lower after the extraction and process. Please well consider the risk of lack of concentration prior start the measurement.
    Our recommendation is more than 2mL blood samples.
27106
Rat Intact Angiotensinogen Assay Kit - IBL
  • Question
    Q.Can urine samples be used for this kit?
  • Answer
    A.No, it cannot.
27105
Mouse Intact Angiotensinogen Assay Kit - IBL
  • Question
    Q.Can urine samples be used for this kit?
  • Answer
    A.No, it cannot.
27104
Rat Total Angiotensinogen Assay Kit - IBL
  • Question
    Q.Can urine samples be used for this kit?
  • Answer
    A.No, it cannot.
27103
Mouse Total Angiotensinogen Assay Kit - IBL
  • Question
    Q.Can urine samples be used for this kit?
  • Answer
    A.No, it cannot.
27177
Endothelin-1 (1-31) Assay Kit - IBL (Product On Request)
  • Question
    Q.The specificity on the datasheet is described as "Endothelin-1 is ≦0.1％".
    What does exactly mean of "Endothelin-1" described here?
  • Answer
    A.It means Endothelin-1(1-21).
  • Question
    Q.How should I conduct Endothelin extraction process?
  • Answer
    A.Please refer to the datasheet enclosed the product package and/or the datasheet listed on the product page.
    As several publications can be found on the page (product description), please refer to them.
    https://www.ibl-japan.co.jp/en/search/product/detail/id=3873
  • Question
    Q.Is extraction process essential for blood samples?
  • Answer
    A.Yes, it is essential. Please follow the datasheet for the extraction process using column such as SepPak.
  • Question
    Q.I have only 0.5mL blood samples. Is it possible to measure it using this ELISA?
  • Answer
    A.Yes, it is possible, however, in general speaking, the concentration of Endothelin in blood sample is low conc., the concentration could be much lower after the extraction and process. Please well consider the risk of lack of concentration prior start the measurement.
    Our recommendation is more than 2mL blood samples.
27113
Human CTP (Cochlin-tomoprotein) Assay Kit - IBL
  • Question
    Q.Which domain is recognized?
  • Answer
    A.It is LCCL domain.
27900
Human Titin N-Fragment (Urine) ELISA Kit – IBL
  • Question
    Q.If urine samples are not measured immediately after collection, how should they be stored?
  • Answer
    A.They should be store at -20°C or below immediately after collection.
  • Question
    Q.Is any normalization needed for measured value of urine sample?
  • Answer
    A.Yes, it is needed.
    Please measure urinary creatinine at the same time and normalize to it.
  • Question
    Q.Can rodent (mouse or rat) sample be used for this kit?
  • Answer
    A.No, it cannot.
  • Question
    Q.How many time freeze-thaw can be repeated for human urine sample for the assay?
  • Answer
    A.It is stable up to 3 times freeze-thaw repeat. Please refer to the freeze-thaw stability test result from this link.
  • Question
    Q.Do you have any storage (temperature) stability data of urinary titin N-terminal fragment (UTF)?
  • Answer
    A.Yes, we do. Please refer to the data.
27142
Human GROβ Assay Kit- IBL
  • Question
    Q.Is GROβ same as CXCL2?
  • Answer
    A.Yes, it is.
27418
Human Amyloidβ (N3pE-40) Assay Kit - IBL
  • Question
    Q.Do you have any protocol for preparation of mice and/or rat brain
    tissue extracts?
  • Answer
    A.CHAPS is recommended for extractin of wild type (W.T.) mice or rat brain tissue.
    Please refer to the following link for the brain tissue extraction process using CHAPS.
    http://www.ibl-japan.co.jp/files/topics/4944_ext_02_0.pdf

    Guanidine solution is recommended for extraction of Transgenic (Tg) mice or rat brain tissue.
    Please refer to the following link and the following two scientific articles for brain tissue extraction process using Guanidine.
    http://www.ibl-japan.co.jp/files/topics/4945_ext_02_0.pdf

    Presenilin-2 mutation causes early amyloid accumulation and memory impairment in a transgenic mouse model of Alzheimer's disease. Toda T et al. J Biomed Biotechnol. 2011;2011:617974.
    https://www.ncbi.nlm.nih.gov/pubmed/?term=21234330

    Methylene blue modulates β-secretase, reverses cerebral amyloidosis, and improves cognition in transgenic mice. Mori T et al. J Biol Chem. 2014 Oct 31;289(44):30303-17.
    https://www.ncbi.nlm.nih.gov/pubmed/?term=25157105

    * The ELISA kit #27712 Aβ42 and #27714 Aβ40 have been upgraded to #27719 Aβ (1-42)(FL) and #27718 Aβ (1-40)(FL).
  • Question
    Q.What peptide is used for Standard enclosed in the kit (27418)?
  • Answer
    A.It's synthetic peptide N3pE 3-40.
27716
Human Amyloidβ (N3pE-42) Assay Kit - IBL
  • Question
    Q.Do you have any protocol for preparation of mice and/or rat brain
    tissue extracts?
  • Answer
    A.CHAPS is recommended for extractin of wild type (W.T.) mice or rat brain tissue.
    Please refer to the following link for the brain tissue extraction process using CHAPS.
    http://www.ibl-japan.co.jp/files/topics/4944_ext_02_0.pdf

    Guanidine solution is recommended for extraction of Transgenic (Tg) mice or rat brain tissue.
    Please refer to the following link and the following two scientific articles for brain tissue extraction process using Guanidine.
    http://www.ibl-japan.co.jp/files/topics/4945_ext_02_0.pdf

    Presenilin-2 mutation causes early amyloid accumulation and memory impairment in a transgenic mouse model of Alzheimer's disease. Toda T et al. J Biomed Biotechnol. 2011;2011:617974.
    https://www.ncbi.nlm.nih.gov/pubmed/?term=21234330

    Methylene blue modulates β-secretase, reverses cerebral amyloidosis, and improves cognition in transgenic mice. Mori T et al. J Biol Chem. 2014 Oct 31;289(44):30303-17.
    https://www.ncbi.nlm.nih.gov/pubmed/?term=25157105

    * The ELISA kit #27712 Aβ42 and #27714 Aβ40 have been upgraded to #27719 Aβ (1-42)(FL) and #27718 Aβ (1-40)(FL).
  • Question
    Q.What peptide is used for Standard enclosed in the kit (27716)?
  • Answer
    A.It's synthetic peptide N3pE 3-42.
27718
Human Amyloidβ (1-40) (FL) Assay Kit - IBL
  • Question
    Q.Do you have any protocol for preparation of mice and/or rat brain
    tissue extracts?
  • Answer
    A.CHAPS is recommended for extractin of wild type (W.T.) mice or rat brain tissue.
    Please refer to the following link for the brain tissue extraction process using CHAPS.
    http://www.ibl-japan.co.jp/files/topics/4944_ext_02_0.pdf

    Guanidine solution is recommended for extraction of Transgenic (Tg) mice or rat brain tissue.
    Please refer to the following link and the following two scientific articles for brain tissue extraction process using Guanidine.
    http://www.ibl-japan.co.jp/files/topics/4945_ext_02_0.pdf

    Presenilin-2 mutation causes early amyloid accumulation and memory impairment in a transgenic mouse model of Alzheimer's disease. Toda T et al. J Biomed Biotechnol. 2011;2011:617974.
    https://www.ncbi.nlm.nih.gov/pubmed/?term=21234330

    Methylene blue modulates β-secretase, reverses cerebral amyloidosis, and improves cognition in transgenic mice. Mori T et al. J Biol Chem. 2014 Oct 31;289(44):30303-17.
    https://www.ncbi.nlm.nih.gov/pubmed/?term=25157105

    * The ELISA kit #27712 Aβ42 and #27714 Aβ40 have been upgraded to #27719 Aβ (1-42)(FL) and #27718 Aβ (1-40)(FL).
27729
Human Amyloidβ (1-ｘ) Assay Kit - IBL
  • Question
    Q.Do you have any protocol for preparation of mice and/or rat brain
    tissue extracts?
  • Answer
    A.CHAPS is recommended for extractin of wild type (W.T.) mice or rat brain tissue.
    Please refer to the following link for the brain tissue extraction process using CHAPS.
    http://www.ibl-japan.co.jp/files/topics/4944_ext_02_0.pdf

    Guanidine solution is recommended for extraction of Transgenic (Tg) mice or rat brain tissue.
    Please refer to the following link and the following two scientific articles for brain tissue extraction process using Guanidine.
    http://www.ibl-japan.co.jp/files/topics/4945_ext_02_0.pdf

    Presenilin-2 mutation causes early amyloid accumulation and memory impairment in a transgenic mouse model of Alzheimer's disease. Toda T et al. J Biomed Biotechnol. 2011;2011:617974.
    https://www.ncbi.nlm.nih.gov/pubmed/?term=21234330

    Methylene blue modulates β-secretase, reverses cerebral amyloidosis, and improves cognition in transgenic mice. Mori T et al. J Biol Chem. 2014 Oct 31;289(44):30303-17.
    https://www.ncbi.nlm.nih.gov/pubmed/?term=25157105

    * The ELISA kit #27712 Aβ42 and #27714 Aβ40 have been upgraded to #27719 Aβ (1-42)(FL) and #27718 Aβ (1-40)(FL).
27362
Human Mac-2 binding protein (Mac-2bp) Assay Kit - IBL
  • Question
    Q.What is the epitope of antibodies used in this kit?
  • Answer
    A.Anti-Human Mac-2bp (8A2) Mouse IgG (Precoated Antibody) recognizes position no. 182-276 amino acid of human Mac-2bp.
    Anti- Human Mac-2bp (67A1) Mouse IgG (HRP Antibody) recognizes position no. 361-440 amino acid of human Mac-bp.
  • Question
    Q.What is the immunogen of 2 antibodies used in the kit?
  • Answer
    A.Recombinant protein was used as the immunogen for both antibodies.
27710
Human Amyloidβ (1-43) (FL) Assay Kit - IBL
  • Question
    Q.Can this kit be used for mouse or rat samples?
  • Answer
    A.No, it cannot.
    A monoclonal antibody (82E1) which is used as the labeled antibody in this kit, specifically reacts to N-terminal of Human Aβ.
    Since the N-terminal sequence of Aβ is different between human and rodents (mouse or rat), it does not cross reacts to mouse and/or rat Aβ.
27259
Mouse Osteopontin N-Half Assay Kit - IBL
  • Question
    Q.Can this kit be used for bovine samples?
  • Answer
    A.No, it cannot.